Sperm retrieval by masturbation is usually done by the male partner in IVF. For those who have difficulty in sperm retrieval, sperm can be retrieved under the psychological guidance of a male physician and pharmacological intervention. If these modalities are still ineffective, percutaneous epididymal and testicular puncture sperm retrieval can be performed, while in azoospermic individuals undergoing IVF, epididymal and testicular puncture sperm retrieval or microscopic testicular sperm retrieval is used to retrieve sperm. The purpose of surgical sperm retrieval is to obtain sufficient number of sperm for intracytoplasmic single sperm microinjection, also known as second generation IVF, in order to obtain a high fertilization rate and achieve pregnancy. If microscopic sperm retrieval fails, only IVF with sperm bank donor can be done. There is also a possibility that men with difficulties in sperm retrieval can also freeze their sperm in advance. Some people are able to masturbate and take out their sperm on the day of egg retrieval because they are in a relaxed mood.