The five sex hormones include estradiol, follicle stimulating hormone, luteinizing hormone, testosterone, and prolactin. Estradiol and testosterone are secreted by the ovaries, while follicle stimulating hormone, luteinizing hormone, and prolactin are secreted by the hypothalamus. The results are more accurate because this period is in the early follicular phase, which represents the basal hormone level of a person, and is more helpful for evaluating ovarian function and diagnosing female endocrine-related diseases, such as irregular menstruation, amenorrhea, infertility, miscarriage and other related diseases, if Women who have not had their periods for a long time can always have their blood drawn for the test. Women should not take estrogen or short-acting contraceptive pills one to two months before the test to avoid affecting the test results.