Morning urine on the seventh day of blastocyst is not always detectable. Blastocyst transfer refers to the technique of in vitro fertilization-embryo transfer, which is the process of fertilization of sperm and egg outside the body and cultivation and development to the stage of blastocyst, and then transferring to the uterine cavity and enabling it to implant and develop into a fetus, which is operated by a professional doctor. Generally, a urine pregnancy test is performed 2 weeks after the blastocyst transfer to confirm pregnancy, and a morning urine test on the seventh day after the transfer may not always detect pregnancy. After blastocyst transfer, you must pay attention to proper rest, avoid strenuous exercise and labor, prohibit coitus, eat a light diet, avoid spicy stimulation and cold food. Also follow the doctor’s advice to continue luteal support therapy. Generally, ultrasound examination can be carried out 4~5 weeks after the transplantation to confirm normal intrauterine pregnancy.