The normal value of total bile acids in newborns may vary slightly from hospital to hospital due to the different reagents and measuring instruments used, but the overall difference is not great. Total bile acids are mainly composed of bile acids, goose deoxycholic acid and deoxycholic acid, lithobiliary acid and ursodeoxycholic acid produced in metabolism. Total bile acids can reflect the liver function well. When hepatocellular damage or intra- or extrahepatic bile duct obstruction occurs, bile acids will be significantly abnormal and total bile acids will be significantly elevated. For example, acute and chronic severe hepatitis, common causes include viral hepatitis B, autoimmune hepatitis, EBV hepatitis, cytomegalovirus hepatitis, and may be caused by biliary lesions such as cholestatic cirrhosis, primary sclerosing cholangitis, congenital biliary atresia, and congenital bile duct dilatation. For clinical abnormalities of total bile acids, it is necessary to combine with relevant imaging and other auxiliary examinations to further determine the specific cause of the increased bile acids and provide timely treatment.