Syphilis toluidine red serologic test 1:16 suggests that there may be active syphilis spirochetes in the body, but whether it is serious or not needs to be combined with the patient’s syphilis-specific serologic test and clinical symptoms to make a comprehensive judgment. Toluidine red unheated serologic test for syphilis, TRUST, is a non-syphilis spirochete antigen test, is a non-specific test, the titer of normal people should be negative, the result of the test is 1:16 is an elevated antibody titer, which indicates that there may be active syphilis spirochetes in the body. However, the test alone cannot confirm the diagnosis of syphilis. Leprosy, tuberculosis, infectious mononucleosis, lupus erythematosus, pregnant women and other groups can also appear syphilis toluidine red serologic test positive, it is recommended to further improve the syphilis specific serologic test, both positive can be diagnosed as syphilis. Therefore, there is no need to be too nervous about this result, and it is recommended to improve the syphilis-specific serologic test in order to clarify the diagnosis as soon as possible. The result is not too nervous.