Antinuclear antibody 1:160 suggests a positive antinuclear antibody, and autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and dry syndrome need to be considered. The titer of antinuclear antibody in a normal person should be less than 1:40, so an antinuclear antibody titer of 1:160 is positive. This antibody is associated with autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, desiccation syndrome, systemic sclerosis, and primary biliary cirrhosis, but the final diagnosis needs to be made in the context of the patient’s symptoms, history, and other ancillary findings, and to rule out other diseases. In addition to autoimmune diseases, antinuclear antibodies are also elevated in the elderly, patients with chronic diseases, and patients with tumors. Therefore, only positive antinuclear antibodies cannot confirm the diagnosis of the disease, and the patient needs to further improve the antinuclear antibody karyotype, other autoimmune antibodies, rheumatoid factor, blood sedimentation, and other auxiliary tests. Laboratory results should be interpreted by a professional physician, and it is recommended that patients consult the rheumatology and immunology department of a regular hospital.