Negative Mycoplasma pneumoniae suggests that there is no infection with Mycoplasma pneumoniae for the time being, or there is a false-negative possibility during the incubation period of the infection. Mycoplasma pneumoniae is a common infectious agent in the respiratory system, which can cause fever, cough, fatigue, headache and other symptoms, coughing is mostly persistent paroxysmal severe dry cough, heavy at night. In the clinic, we can take the respiratory tract, urethra and other parts of the secretions for culture, to determine whether there is mycoplasma growth; or through the antigen and antibody IgM test, if there is a Mycoplasma pneumoniae infection, the results are generally positive, but may also be in the incubation period of the infection, and the possibility of false-negative. In the examination of Mycoplasma pneumoniae, the result is negative does not necessarily mean that there is no Mycoplasma pneumoniae infection, but also need to synthesize the clinical symptoms and other related laboratory tests to determine the comprehensive, during the period of respiratory symptoms should be timely to go to the hospital, in order to avoid delaying the condition.