Edema and congestion of the esophageal mucosa is caused by esophagitis. Esophagitis (esophagitis) is an inflammation of the superficial or deep tissues of the esophageal mucosa due to abnormal irritation and edema and congestion of the esophageal mucosa. Common tests: 1. Leukocyte count The surface layer of blood is grayish white when centrifuged, and the cells in this part are called leukocytes. It is a collective term for a group of non-homogeneous mixed cells with different morphology, function and stages of development and differentiation, which are classified into three categories: granulocytes, lymphocytes and monocytes according to their morphology, function and origin. The clinical significance of leukocyte count alone is limited and should be combined with the leukocyte classification count to analyze the condition more precisely. Role of the test: Leukocyte count can reflect the body’s defense system, and is useful for the diagnosis and treatment of infectious diseases. Examination procedure: (1) Take a small test tube and add 0.38ml of leukocyte diluent. (2) Use a hemoglobin pipette to accurately draw 20μl of peripheral blood. (3) Wipe off the remaining blood outside the tip of the tube, insert the pipette into the bottom of the test tube containing 0.38ml of diluent, gently blow out the blood, and suck out the supernatant and rinse 3 times, taking care not to mix the diluent each time, and finally shake the test tube by hand to mix. (4) Fill the liquid, wipe the counting pool and coverslip, cover the coverslip on the counting pool, then use the micropipette to quickly absorb the mixed suspension to fill the counting pool, and let it stand for 2~3min after microscopic examination. (5) Count the total number of leukocytes in the four corners of the four large squares with a low magnification microscope. For the leukocytes in the pressure line, the principle of counting up but not down and counting left but not right should be adopted to ensure the consistency and accuracy of the counting results in the counting area. 2, hepatobiliary imaging, that is, intravenous injection of hepatobiliary imaging agent, can be taken up by hepatocytes, then secreted into the capillary bile ducts, and then discharged to the intestine through the biliary system, the use of γ camera can be dynamically observed. The role of examination: hepatobiliary imaging has an important role in the diagnosis of acute cholecystitis. Acute cholecystitis is often associated with inflammatory edema of the bile ducts, resulting in mechanical or functional complete obstruction. In intrahepatic and extrahepatic bile duct obstruction, thickening or cystic dilatation of the proximal bile duct is seen; no radioactivity is seen in the intestine of hepatobiliary imaging in complete obstruction of the common bile duct, and delayed appearance of intestinal radioactivity in incomplete obstruction. Examination procedure: (1) Imaging method The patient is placed in the supine position, using a large field of view, low-energy universal collimator; the image is acquired immediately after injection of the imaging agent, and dynamic imaging is performed at 5, 10, 20, 30, 45 and 60 min, respectively. If acute cholecystitis is highly suspected and the gallbladder is not visualized in 60 minutes, a 3-4h delayed image should be added; certain lesions, such as common bile duct and bile duct stenosis, should be delayed in 18-24 hours. (2) Interventional tests ① Cholecystokinin (CCK); synthetic cholecystokinin Sincalide has the activity of pro-cholecystokinin. The dose of 0.2μg/kg, the gallbladder began to contract after slow injection, and the effect reached its peak in 15min. the role of Sincalide: First, it is used to empty the gallbladder of bile before the examination for those who have fasted for more than 24h. The second is to measure the contractile function of the gallbladder. Morphine can be used to shorten the time needed to confirm the diagnosis of acute cholecystitis. The injection dose is 0.04mg/kg, and the maximum dosage is 2-3mg. It is suitable for those who have high suspicion of acute cholecystitis and the gallbladder is not visualized for 45-60min and there is no evidence of total bile duct obstruction. ③Fat meal test (fatdiettest); measurement of gallbladder contractile function by eating fat meals or other fat stimulation. ④Phenobarbital (barbital); when infant jaundice is suspected, luminal 5mg/kg is usually given orally in two divided doses daily for at least 3-5 days to increase hepatobiliary excretion of the developer and to improve the specificity of the diagnosis of congenital biliary atresia.