Thyroid nodule grade 4A may require a thyroid function pentathlon, which is recommended for examination and treatment under the supervision of a doctor. Thyroid nodule grade 4A is the result depicted by thyroid ultrasound, which suggests a low suspicion of malignancy, with a malignancy rate of 2% to 10%, and may require a thyroid function pentathlon to understand the function of the thyroid gland. In addition to benign thyroid nodules, grade 4A thyroid nodules may also be malignant thyroid nodules, such as thyroid cancer, which may also affect thyroid function, so it is necessary to have a thyroid function penta-screening test, which also facilitates the next step of treatment if the problem is detected in time. Thyroid function screening includes T3, T4, TSH, anti-thyroglobulin antibodies, and thyroid peroxidase antibodies. In order to distinguish the nature of thyroid nodules, in addition to the thyroid function five items, if necessary, can follow the doctor’s instructions for pathological biopsy, and then according to the diagnosis of the results of targeted treatment to avoid the development of the disease.