The main causes of herniated discs leading to back and leg pain are twofold: mechanical compression of nerves, aseptic inflammation of nerve roots and nutritional disorders of nerve roots and conduction damage of nerve roots. The principle of medical ozone treatment for intervertebral discs: 1, oxidation of proteoglycans, destruction of nucleus pulposus cells, ablation Medical ozone has strong oxidizing ability, injecting it into the nucleus pulposus of the herniated disc, it can oxidize the proteoglycans in the nucleus pulposus within a short time, and at the same time, the nucleus pulposus cell membrane and intracellular structure are destroyed, the function of cell synthesis and secretion of proteoglycans is decreased or lost, and the osmotic pressure of the nucleus pulposus tissue is reduced. This leads to loss of water and atrophy and reduction in the volume of the nucleus pulposus, thereby reducing the pressure in the intervertebral disc and eliminating the symptoms and achieving the purpose of treatment. Among the tissues constituting the cartilage end plate, fibrous ring and nucleus pulposus of the intervertebral disc, the content of proteoglycans in the nucleus pulposus is the highest, reaching 40-60% of the dry weight of the nucleus pulposus; medical ozone concentration at 40ug/ml can effectively oxidize and decompose proteins and polysaccharide macromolecules in the nucleus pulposus, but the effect on the cartilage end plate and fibrous ring is weak. 2, medical ozone has anti-inflammatory effect The prominent nucleus pulposus and fibrous ring compress the dura mater, nerve roots and surrounding veins, causing impaired reflux, exudation and tissue edema; glycoprotein and β-protein released after fibrous ring fracture as antigenic substances, causing the body to produce an immune response. Medical ozone can stimulate the overexpression of oxidase, neutralize the overproduction of reactive oxidation products in the inflammatory response, antagonize the release of immune factors in the inflammatory response, dilate blood vessels, improve reflux, and reduce edema around nerve roots. 3, medical ozone has analgesic effect The protruding intervertebral disc tissue can compress the nerve roots and stimulate the release of pain-causing substances, such as substance P, phospholipase A2, etc., from the small intervertebral joint protrusions, adjacent ligaments and nerve endings present on the surface of the intervertebral disc, thus producing pain. Medical ozone injection can act directly on the above-mentioned nerve endings and stimulate the release of substances such as enkephalins from inhibitory interneurons, thus achieving analgesic effects. 4.Collagenase is an enzyme that mainly dissolves collagen, which can effectively dissolve collagen fibers in the nucleus pulposus and the fibrous ring, which dissolves type I and II collagen fibers, destroys the proteoglycan-attached mesh structure, affects the hydrophilic property of proteoglycan and causes water loss, and activates endogenous enzymes in the nucleus pulposus to decompose proteoglycan. The two effects cause the pressure in the disc to decrease, eliminating the compression and stimulation of the nerve root and dural sac by the herniated disc, thus achieving the clinical treatment effect. The combined application of the two methods has the following advantages: firstly, ozone is injected into the disc to oxidize part of the nucleus pulposus tissue, which reduces the density of the nucleus pulposus and intra-disc pressure and provides an effective space for intra-disc injection of collagenase. Later, ozone and collagenase are injected within the herniation to dissolve the herniated disc tissue, acting in both directions. In addition, ozone diffuses to the surface of the herniated disc and around the nerve root through the fissure of the annulus fibrosus and the escape of the intra-herniated injection, which can play a role in decomposing adhesions and anti-inflammatory and pain relief, thus achieving the treatment purpose. It avoids the shortcomings such as the increase of back pain within 1 week after collagenase nucleolysis treatment alone and the unstable long-term efficacy of medical ozone treatment alone. Indications (1) Lumbar disc herniation 1, lumbar and leg pain, claudication, abnormal sensation and leg pain more than lumbar pain and other clinical symptoms are obvious; 2, there are signs of spinal nerve compression, such as straight leg raise test, lumbar spine flexion and extension test, foot dorsal extension/thumb dorsal extension and reduced mechanical force; 3, clinical symptoms and signs are consistent with CT/MRI imaging diagnosis; 4, ineffective or repeated after 3 months of conservative treatment. (2) Cervical disc herniation 1. cervical shoulder and arm pain and numbness, cervical back and scapular pain, cervicogenic vertigo/headache; 2. cervical nerve/vertebral artery compression signs, such as positive spinal canal/intervertebral foramen compression test, positive cervical rotation provocation test, reduced biceps muscle strength/thumb to palm function; 3. clinical symptoms and signs consistent with CT/MRI imaging diagnosis; 4. recurrent symptoms, ineffective after Conservative therapy is ineffective for a long time.