Sperm is usually removed by masturbation or by sexual intercourse followed by in vitro ejaculation. If sperm cannot be removed through these methods, it is recommended that a testicular puncture biopsy be performed to look for mature sperm directly from inside the testicular tissue. The sperm will be taken out and mainly used for semen analysis examination. This examination is an important indicator to determine male fertility, including many examination items, such as sperm vitality, sperm motility rate, sperm liquefaction time, abnormality rate, etc., which can initially determine the quality of male sperm and provide reference for the fertility of the patient.