The quantification of hepatitis B surface antigen is very significant because the specific quantification of hepatitis B surface antigen can indirectly reflect the replication of hepatitis B DNA and the amount of hepatocyte cccDNA. If the quantification of hepatitis B surface antigen is high, it indicates that hepatitis B virus replication is very active and the amount of hepatitis B virus cccDNA is high, the possibility of achieving a negative hepatitis B surface antigen in a short period of time, or the possibility of changing from major triplet to minor triplet is even lower, which is prospective for the treatment of hepatitis B. In the course of hepatitis B treatment, it is also necessary to conduct regular rechecking of hepatitis B surface antigen quantification, which can reflect the active level of hepatitis B virus replication in the body according to the change in the level of hepatitis B surface antigen quantification.