The first test tube examination requires physical examination, infertility examination, hysteroscopy, male reproductive endocrine examination and so on. 1. Physical examination (for both husband and wife): it includes general examination, weight, growth and development, thyroid gland, breast gland, development of secondary sexual characteristics, etc. In addition, blood and urine routine, liver and kidney function, Hepatitis B 5, AIDS, syphilis and other diseases need to be examined. In addition, we need to check blood and urine routine, liver and kidney function, Hepatitis B 5, AIDS, syphilis, coagulation four, blood sedimentation and so on. 2. Infertility examination: gynecological ultrasonography, double diagnosis examination, tubal patency examination, male urogenital ultrasonography. To know whether the reproductive tract is blocked or not. 3. Hysteroscopy: to determine whether there are lesions in the pelvis, such as endometriosis foci, tubal lesions, ovarian lesions and so on. 4. Male external genitalia, reproductive endocrinology, semen analysis: to find out whether there are any genital malformations, the function of hypothalamus-pituitary-testicular axis, sperm count, motility, survival rate and morphology. It is recommended that couples with IVF needs should go to a professional reproductive medicine center for treatment, and choose the examination method according to the conditions of both parties under the guidance of the doctor.