Anti-CCP antibodies are anti-cyclic citrullinated polypeptide antibodies. Negative anti-CCP antibodies do not prove the absence of rheumatoid arthritis, but need to be combined with symptoms and test results to determine. Anti-cyclic citrullinated polypeptide antibody is a kind of autoantibody, which is often positive in patients with rheumatoid arthritis, and helps to diagnose rheumatoid arthritis, but there are also some diagnosed patients with negative anti-cyclic citrullinated polypeptide antibody. Therefore, a negative CCP does not rule out a diagnosis of rheumatoid arthritis. The diagnosis of rheumatoid arthritis also requires a combination of symptoms and other test results. Rheumatoid arthritis is often characterized by symmetrical swelling and pain in multiple joints of the hands, wrists, and feet, as well as extra-articular manifestations such as rheumatoid nodules on the skin and rheumatoid vasculitis, etc. Abnormalities in C-reactive protein and sedimentation rate may indicate disease activity, and a positive rheumatoid factor may also assist in the diagnosis. When rheumatoid arthritis is suspected, it is necessary to actively consult the rheumatology and immunology department and follow the doctor’s instructions for reasonable treatment.