Anti-Extractable Nuclear Antigen (ENA) is a diagnostic test for autoimmune diseases. ENA can be extracted from the nucleus of cells in saline or phosphate buffer, and mainly includes Sm, U1-RNP, SSA, SSB, Jo-l, and Scl-70, etc. Antibodies directed against these antigens are anti-Extractable Nuclear Antigen (ENA) antibodies. ENA is a general term for extractable nuclear antigen, which can be extracted from the nucleus of a cell in saline or phosphate buffer.ENA is a non-histone nuclear protein, an acidic protein antigen, a ribonucleoprotein particle consisting of many small molecules of RNA with their respective counterparts of specific proteins, which enhance their respective antigenicity, and a molecule that does not contain DNA. ENA antigens mainly include Sm, Ul-RNP, SSA, SSB, Jo-l and Scl-70 antigens, etc. Different autoimmune diseases can produce different anti-ENA antibodies, according to the molecular weight of ENA antigens and different antigenic properties, different immunological methods can be used to detect these autoantibodies, and the most commonly used method at present is immunoblotting technology. The most commonly used method is the immunoblotting technique. If you need to perform anti-Enhanceable Nuclear Antigen (ENA) antibody measurement, it is recommended that you go to the hospital and consult your doctor for the results.