Factors affecting the enzymatic reaction include the concentration of enzyme, the concentration of reaction substrate, temperature and pH, inhibitor and activator.
1. Concentration of enzyme: when other influencing factors are certain, the higher the concentration of enzyme, the faster the speed of enzymatic reaction, that is to say, the speed of reaction is proportional to the concentration of enzyme.
2. Concentration of reaction substrate: when other factors are certain, the reaction speed and substrate concentration into a rectangular hyperbola, that is to say, when the concentration of substrate is very low, the reaction speed is proportional to the concentration of substrate, when the concentration of substrate continues to increase, the increase of reaction speed gradually slows down, and reaches the maximum reaction speed, with the increase of substrate concentration, the reaction speed is no longer increased.
3. Temperature and pH value: Enzymatic reaction has optimal temperature and optimal pH value, in the optimal temperature and optimal pH value, the reaction speed is the fastest, too high or too low will slow down the reaction speed.
4. Inhibitors and activators: substances that inhibit or activate enzymatic reactions.