There are differences between first and second generation IVF, both in terms of indications and operation. First of all, the indications are that first-generation IVF is mainly for moderate and some severe male oligospermia and mild to moderate malformed spermatozoa, while the indications for the female partner include repeated IUI failure, endometriosis, polycystic ovarian syndrome, tubal obstruction, etc. Second-generation IVF is a supplement and derivative of the first-generation IVF technology, mainly for men with severe oligospermia, obstructive azoospermia and severe malformed spermatozoa, as well as patients with repeated IVF fertilization failures. Secondly, in terms of technical operation, 1st generation IVF is to place the sperm and egg in a culture fluid and combine them in their natural state to complete the fertilization process and the later division process. In second generation IVF, sperm with better morphology and vitality are artificially selected and injected into the cytoplasm of the egg to complete the fertilization process, followed by the same division process as in first generation IVF. There is no significant difference between the two in terms of pregnancy rate, embryo division process, percentage of high quality embryos, and safety of the offspring.