I. Application form for routine pathological examination 1. Histopathology: puncture tissue, endoscopic tissue, locally excised tissue, surgical specimens. Time for result: the third working day after receiving specimens. 2. Cytopathology: sputum, brushings, bronchoscopic lavage, cervical scrapings, pleural fluid, ascites, pericardial effusion, puncture cell smear, etc. Note with sputum specimen retention: patients are instructed to send the second sputum from deep trachea or bronchus after gargling early in the morning for examination. Time of result: the second working day after receiving the specimen. 3.Frozen pathology: The clinician will notify the pathology department by phone before the operation, and the results will be available about 40 minutes after the specimen is delivered to the pathology department. 4.General pathology consultation: directly fill out the application form and bring the pathology slides to the pathology department. The result time: the same day. 5.Remote consultation: directly fill out the application form and bring the pathology slides to the pathology department. Result time: the same day. Usually within 24 hours, special cases will be notified in advance. 6.Tumor Abnormal Protein (TAP) test: 40-50 microliters of fingertip blood will be collected by the pathology department to make thick blood film microscopy. Time to result: 24 hours after specimen collection. B. Application for DNA determination of various pathogens 1. Mycobacterium tuberculosis, Mycoplasma pneumoniae: sputum, tracheoscopic lavage, pleural fluid, ascites, pericardial effusion, cerebrospinal fluid, urine, anticoagulated blood, paraffin-embedded tissue, fresh biopsies, pus and secretions, etc. All the above specimens were retained in sterile containers with lids and sent to the pathology department, and anticoagulated blood was taken in 2ml with blue-cap anticoagulation tubes for examination. 2, hepatitis B virus: serum or procoagulant blood. Coagulation blood with yellow cap procoagulation tube to draw 2ml sent for testing. Results time: all within 24 hours after receiving the specimen. C. Flow cytometry test application form Test purpose: to detect the patient’s immune function, which can indicate infectious diseases, immune diseases or malignant tumors; detection of HLA-B27, which is an important indicator of ankylosing spondylitis. Test items: 1, T-lymphocyte subpopulation (3 items) 2, B-lymphocytes 3, NK cells 4, HLA-B27 The above test items to keep the specimen with a purple cap anticoagulation tube to draw 2ml of blood was sent for testing. Results time: all within 24 hours after receiving the specimen. The purpose of the test is to detect pathogens in vaginal secretions. Vaginal secretions are taken with a sterile cotton swab and sent for testing. Time to result: within 20 minutes after receiving the specimen. V. Application for secondary immunotherapy Use CIK cells to improve immunity, correct immune imbalance, effectively inhibit and kill residual tumor cells in the body, and as an adjunct to the treatment of infectious diseases (mainly for malignant tumors and tuberculosis).