Amniocentesis is required for three-generation IVF. Three-generation IVF refers to the assisted reproductive technology that carries out the analysis and judgment of the genetic material of the embryo to determine whether there is any abnormality in the embryo before the embryo is transferred. It is suitable for those who have repeated miscarriages due to abnormalities in the chromosome number or structure of the embryo, or for couples who have genetic disorders of sex chromosome chain. In addition, embryos carrying disease-causing genes are excluded through in vitro genetic diagnosis of embryos. Due to technical limitations and the possibility of chimerism in embryos, it is still not possible to completely screen out all abnormal embryos, and embryos may still have genetic abnormalities. Therefore, amniocentesis is still necessary. Clinically, three-generation IVF is one of the indications for amniocentesis. It is recommended that women who undergo three-generation IVF should undergo obstetrical examination on time to ensure the normal growth and development of the fetus.