Second-generation sequencing refers to the simultaneous subsequent mutation status detection and mutation measurement of multiple mutation sites in multiple genes to guide subsequent targeted therapy. Second-generation sequencing, also known as next-generation sequencing, can simultaneously assess the status of hundreds of thousands to hundreds of thousands of genes at the same time. With the continuous development of the technology, it provides great convenience for subsequent targeted testing and makes up for many previous shortcomings. For example, when performing small biopsy specimens by puncture, it is impossible to have enough tissues for subsequent multiplex PCR testing, and there are not enough tissues for repeated validation of multiple targets for multiple indicators, and second-generation sequencing is a perfect solution to this problem. Second-generation sequencing provides excellent technical support for molecular targeted therapy and subsequent immunotherapy, and the cost is not high. If you want to know more, it is recommended to consult a professional doctor.