Before all surgeries, the operator is required to sterilize the hands aseptically. However, some bacteria in the nail crevices of the fingers and in the skin follicles cannot be completely killed in a short period of time, so it is very necessary to wear gloves during the operation. Wearing gloves during surgery can maximize the protection of the patient’s incision in a sterile state, avoiding the operator’s body surface bacteria, viruses infected patient’s incision. At the same time, the operator wearing gloves has an important protective effect on himself, such as certain AIDS, syphilis, hepatitis B, hepatitis C patients, whose blood has a certain degree of contagious. Wearing gloves can effectively block the spread of blood diseases to themselves. Therefore, the sterile barrier established by wearing gloves has a very important role for both the operator and the patient.