Normal values for myoglobin vary depending on the method of testing. Normal values for myoglobin are serum: (3.5-22.8) ng/mL (ELISA method); (29±16.3) μg/L (RIA method). Myoglobin is an oxygen-containing binding protein specific to cardiac and skeletal muscle tissues, and normal human serum contains very little myoglobin. If cardiac or skeletal muscle is injured, it can lead to elevated levels of myoglobin in the blood. Myoglobin values are valuable in diagnosing acute myocardial infarction and skeletal muscle damage. Elevated myoglobin can be seen in the early stages of acute myocardial infarction, acute muscle injury, myotonic dystrophy, myasthenia gravis, polymyositis, acute or chronic renal failure, severe congestive heart failure and prolonged shock. It can increase 1.5h after myocardial infarction, but normalizes within 1-2d. If the patient’s myoglobin value is abnormal, it is recommended that the patient go to the hospital in a timely manner to improve the relevant examination, to clarify the cause of the disease, and follow the doctor’s instructions for treatment, so as not to delay the condition.