The significance of lung cancer puncture biopsy is to clarify the diagnosis and guide the subsequent treatment. According to the location of lung lesions, lung cancer puncture biopsy is chosen to puncture the tumor under the guidance of CT, ultrasound, etc. to take out part of the tissue samples or cell samples for pathologists to conduct pathology testing, which can diagnose lung cancer, clarify the type of pathology and obtain molecular features and genetics characteristics of the tumor, which can provide the basis for the selection of subsequent treatment plans. Puncture biopsy for lung cancer is one of the most commonly used methods to obtain tumor tissue specimens from the lungs. Pathological examination of the punctured tissue is the gold standard for cancer diagnosis, and is of vital significance for the definitive diagnosis and subsequent treatment of lung cancer.