It is possible to detect pregnancy after 35 days without a period, but it depends on the test used to detect pregnancy. It is more accurate to measure HCG in the blood, also known as human chorionic gonadotropin, because the concentration in the blood is more accurate and sensitive, and the detection of HCG is relatively stable. At this time, if HCG is measured in the urine, it may not give a positive result because the concentration in the urine is much lower than that in the blood, and only when it reaches a sufficient concentration will the urine indicate a positive result. Therefore, for people with regular menstrual cycles, it is better to test the HCG in blood rather than urine HCG if there is no pregnancy for 35 days.