Pressure ulcers, also known as pressure ulcers, are skin defects caused by long-term pressure on local tissues, impaired blood circulation, continuous ischemia and hypoxia, and malnutrition, resulting in tissue ulceration and necrosis, which severely damage the integrity of the skin. At present, the main treatment methods for pressure sores in relevant medical institutions include various types of topical creams for burns combined with antibiotic ointments, various types of epidermal growth factors combined with physical therapy, and clinical research on medical biofilm therapy devices for pressure sores. However, the treatment effect and healing time vary slightly depending on the individual patient, but the optimal pressure sore treatment method has not been reported. Therefore, promoting rapid wound healing, reducing wound scarring and reducing the impact on late function have become the focus of treatment. In this paper, the effect of rb-bFGF combined with amniotic membrane was observed and discussed in comparison with other traditional treatments and simple amniotic membrane treatment without the addition of rb-bFGF for astringency and healing. 1. Materials and methods Forty-six patients, 21 males and 25 females, aged 60-82 years old, average 73.5 years old, admitted from January 2011 to January 2013 were selected; 18 cases of femoral neck fracture, 15 cases of subtrochanteric fracture and 13 cases of lumbar fracture; 40 sacrococcygeal pressure sores, 10 hip sores, 6 heel sores and 7 ankle sores, totaling 63 sores; 25 of them were stage II, stage III The pressure ulcers of stage II and III were selected and randomly divided into 23 cases and 29 cases in the control group and 23 cases and 34 cases in the treatment group. The pressure ulcers were graded according to the following criteria: Stage I with intact skin, but with erythema and discoloration; Stage II with local epidermal loss and damage to the epidermis and dermis, manifesting as skin blisters and ulcers; Stage III characterized by full skin damage, with ulcers reaching deep into the subcutaneous tissue and reaching the muscular layer, mostly accompanied by infection, with mixed yellow, white and gray wounds; Stage IV with extensive involvement of muscles, tendons and bone on the basis of the above, which may be accompanied by osteomyelitis. Sinus tracts can be seen, with much exudate and malodor. Healing: the wound is filled with granulation tissue and the epithelial cells move to the middle and cover the wound. Significant effect: the wound is filled by granulation tissue, epithelial cells move to the middle of the wound, and the diameter of the wound is reduced by more than 1/2 than before. Improvement: The secretion is significantly reduced, the wound is filled with granulation tissue, and the diameter of the wound is more than 1/3 smaller than before. Ineffective: the trabecular surface is still pale, edematous, and has a lot of secretions. Healing, apparent effect and improvement are all considered effective. 2.Materials Recombinant bovine basic fibroblast growth factor (rb-bFGF) trade name: Beifuji, 63000IU/bottle, Zhuhai Yisheng Biopharmaceutical Co., Ltd. sound field, lot number: State Drug Quantifier S10980077., is a multifunctional cell growth factor, with the function of promoting capillary regeneration, improving local blood circulation, accelerating the healing of trauma and actively promoting repair [1 ]. Amniotic membrane assay: The amniotic membrane was obtained from placental tissues of healthy mothers during cesarean section (voluntary donation with signed consent and approved by our hospital ethics committee), and the corresponding serological tests were performed before delivery, thus expelling hepatitis B as well as hepatitis C, syphilis and other infectious diseases, and the placental surface was rinsed with saline under sterile conditions to blunt the amniotic membrane from the chorionic villi. The separated amniotic membrane was soaked in 500 ml of saline containing 800,000 U of gentamicin for 20 min and stored at 4°C in the refrigerator for 8 h for use. Amniotic membrane has the effect of promoting epithelial growth, reducing inflammatory response and inhibiting fibroblast proliferation [2]. 3. Methods Patients with underlying disease anemia and hypoproteinemia were treated with correction of low protein and anemia; diabetic ulcers should be treated with diet control and drug application to stabilize blood glucose first; varicose veins of lower limbs were treated with bed rest and elevation of affected limbs, and high ligation of large and small veins was feasible if ineffective. According to the results of bacterial culture and drug sensitivity test, antibiotics and systemic nutritional support should be applied reasonably and symptomatic treatment should be carried out. In the control group, rb-bFGF was shaken well and sprayed on the wound surface from inside to outside, so as not to drip, and a layer of oil gauze was applied and pressure bandaged; in the treatment group, on the basis of the control group, rb-bFGF was applied to the wound surface after 1 min according to the wound surface. The trauma healing rate, healing time, pain and scar situation and adverse reactions were observed in the two groups after 5d and 10d of treatment, respectively. The visual analogy score (VAS) was used to assess the pain; the modified Vancouver scar measurement method was used to determine the wound scar proliferation. 4. Results 4.163 pressure ulcer wounds with an average of 3 dressing changes resulted in 33 cases of healing; 24 cases were effective; 5 cases improved and healed by flap repair; 1 elderly decubitus ulcer patient died due to cardiopulmonary failure and was designated as ineffective. 4.2 After treatment of stage II pressure ulcer trauma, the trauma healing rate at 5 d increased by an average of 16.8% and the trauma healing time was shortened by an average of 4.11 d compared with the control group, while after treatment of stage III pressure ulcer trauma, the healing rate at 10 d increased by an average of 21.54% and the trauma healing time was shortened by an average of 6.74 d compared with the control group, and the SI was reduced by an average of 2.02. The scar index was significantly lower than that of the control group, (P< 0.05). No significant adverse reactions were observed during treatment in both groups. 5. Discussion Compared with the control group: faster wound healing, shorter healing time, and higher healing rate (P< 0.05); suggesting that under the moist environment formed by rb-bFGF lambda, it can make the wound release and activate the enzymatic clearing effect of protease and urokinase, thus promoting fibrin and necrotic tissue lysis and detachment, while the lysed fibrin in turn acts as a transformation of certain inflammatory cells The lysed fibrin, in turn, acts as a transforming factor for certain inflammatory cells and promotes the secretion of growth factors to accelerate wound healing and shorten the healing time. When used in combination with rb-bFGF, it prevents the loss of growth factors and enables the rapid growth of wound base tissue in a favorable environment. At the same time, it can significantly reduce the scar formation after healing. Compared with traditional treatment methods, it can: (1) rapidly accelerate the process of wound epithelialization and healing; (2) reduce or eliminate wound pain; (3) protect the wound and reduce wound exudation; (4) inhibit bacterial growth and prevent wound infection; (5) reduce wound scar formation; (6) avoid the trauma caused by surgery compared with skin grafting, and is economically safe. Experimentally, it is proved that using, rb-bFGF in combination with amniotic membrane graft not only plays a necessary barrier role in the initial stage of injury, but also provides a good substrate microenvironment for tissue surface using amniotic membrane, and even has antibacterial effect to reduce inflammatory response, thus more favorable for tissue proliferation, differentiation and faster establishment of epithelial barrier. It is a restorative and non-invasive treatment, which is significantly more effective than traditional treatment methods. It is also suitable for the regeneration and repair of skin, nerve, bone and other tissues, and can be widely used for the repair of difficult-to-heal skin wounds and various patients with skin defects that are not suitable for surgery.