The antibody spectrum of myositis usually examined mainly includes anti-Jo-1 antibody, anti-Mi-2 antibody, anti-MDA5 antibody, anti-Ku antibody, etc. Different antibodies have different clinical significance. Patients with myositis/dermatomyositis have a higher chance of being positive for anti-Jo-1 antibodies, and most of them may have symptoms such as interstitial lung disease, joint pain, fever, etc. Clinically, it is mostly seen as anti-Jo-1 antibody syndrome in connective tissue disease. Rash is seen in 95% of anti-Mi-2 antibody body-positive patients, but interstitial lung lesions are rare and have a better prognosis. Anti-MDA5 antibody positivity is common in those with anaplastic dermatomyositis, which often presents with rapidly progressive interstitial pneumonitis and has a poor prognosis. Anti-Ku antibody positive, patients may have muscle weakness, muscle pressure pain, Raynaud’s phenomenon and other symptoms, considered mostly multiple myositis, systemic sclerosis, mixed connective tissue disease and other diseases. It is recommended that patients go to regular hospitals for the examination of anti-myositis antibody, consult the doctor for specific results, and improve other tests to clarify the diagnosis.