Immunohistochemical results of chordoma need tissue antigens expressed by the specimen to determine, generally positive Cyto-K6/7 EMA7/7, CEA6/7, GFAP0/7, etc. suggests a high likelihood of chordoma, and p53 and PCNA positivity often suggests a higher risk of chordoma recurrence. Chordoma originates from embryonic residual chordal tissue, common immunohistochemical indexes include Cyto-K6/7 EMA7/7, CEA6/7, GFAP0/7, Des0/7, α-AT7/7, Lyso4/7, CK, S100, etc., and the positivity of these indexes suggests that chordoma has a high possibility. In addition, some special indicators, such as the size of Ki-67, are related to the proliferation of the tumor, the expression of p53 and PCNA is related to the recurrence of chordoma, and Cath-D and MMP-13 are related to the invasive growth of chordoma. Immunohistochemistry of chordoma varies in different hospitals, and it is recommended that patients go to regular hospitals and be determined according to the specific immunohistochemistry results.