The female endocrine six test is usually recommended to be performed on 2-3 days of menstruation. The female endocrine six test is the sex hormone six test, which includes luteinizing hormone, follicle stimulating hormone, progesterone, androgen, and prolactin. During the 2nd-4th days of a woman’s menstrual cycle, the six hormones in the patient’s body reach their basal levels. Testing during this period can provide insight into the patient’s basal endocrine function status and help in the diagnosis and treatment of the disease. The hormone levels of the menstrual cycle have a certain pattern, and in larger hospitals, different periods of testing the hormone levels in the body will have their corresponding normal reference ranges, so for patients with amenorrhea or those who are eager to know the results of the test, the test can be performed at any time. In addition, it is important to tell the attending doctor what stage of the menstrual cycle you are in before the test so that the doctor can make a clear diagnosis of the disease and give a symptomatic treatment plan based on the test results.