Transurethral vesiculoscopy is a technique that uses a rigid F5-7.3 ureteroscope to retrograde along the normal seminal tract to examine the seminal tract and to diagnose and treat the corresponding diseases. Seminal tract diseases are common clinical pathologies of the reproductive system, mainly including seminal vesiculitis with severe chronic prostatitis, hematospermia, azoospermia due to ejaculatory duct obstruction, seminal vesicle tuberculosis, seminal cysts, congenital malformation of seminal vesicles, seminal vesicle stones or calcification, seminal vesicle tumors, etc. Because some patients with sperm duct disease do not have obvious symptoms and because they occur in conjunction with the more common chronic prostatitis, they are often underappreciated clinically. The spermatic tract disease can present with significant pelvic floor pain, post-ejaculatory pain, non-ejaculation, intractable urinary frequency, urinary urgency, painful urination, hematuria, intractable hematospermia, difficulty in urinating or urinating, etc. Some of the symptoms are similar to prostatitis, so physicians treat them as prostatitis only. However, there are some patients with severe seminal vesicle disease in clinical practice, where conventional conservative treatment is ineffective and further therapeutic measures are needed. Transurethral vesiculoscopy is a technique that allows biopsy of suspicious tissues, lithotripsy of seminal vesicle stones, incision of ejaculatory duct obstruction or cysts, electrodesiccation and cautery of inflammatory changes, and seminal vesicle irrigation for intractable hematospermia while examining. This technique has been carried out in our department, especially for patients with intractable hematospermia, seminal vesicle stones, ejaculatory duct obstruction or cysts, with good results of seminal vesicle flushing, stone removal and ejaculatory duct incision. Spermoscopy is a minimally invasive treatment technique that is less invasive, safe, effective and has fewer complications.