In recent years, with the development of testicular puncture and micro-sperm extraction technology, it has brought hope for fertility to patients with severe oligozoospermia and weak spermatozoa and patients with azoospermia. Sperm bank testicular tissue, sparse sperm cryopreservation technology as a testicular puncture, micro sperm extraction follow-up service technology for patients to provide a guarantee of fertility success rate. In this article, we will briefly introduce testicular tissue and sparse sperm cryopreservation to you from the following four aspects. I. What is testicular tissue, sparse sperm cryopreservation technology? Testicular tissue and sparse sperm cryopreservation is a kind of auxiliary reproductive technology service provided by human sperm bank for the treatment of infertility caused by male reproductive dysfunction; it is mainly a technical method to cryopreserve testicular tissue and sparse sperm obtained after testicular puncture, micro sperm extraction and other surgeries, and to assist in the birth of progeny by using single-sperm injection technology after thawing and recovery. Is there any risk of testicular tissue and sparse sperm cryopreservation? The core technology of testicular tissue and sparse sperm cryopreservation is freeze-thaw technology. Freezing and thawing technology is to reduce the metabolism of sperm cells under artificially low temperature and make them dormant, and then give them the right temperature at the right time to “wake up” and restore their reproductive function. During the freezing and thawing process, spermatozoa will experience a certain degree of damage, but this damage may vary greatly among individuals. According to relevant data, the current combined success rate of testicular puncture and microscopic sperm extraction is about 40%. C. What procedures are required to apply for testicular tissue and sparse sperm cryopreservation? Testicular tissue and sparse sperm cryopreservation belongs to the self-sperm cryopreservation for the purpose of assisted reproduction, and the applicants for self-sperm cryopreservation for the purpose of assisted reproduction should be couples in accordance with the Marriage Law of the State, and the requirement of reproduction is in accordance with the national family planning policy. The application should provide the original identity card, household registration certificate and marriage certificate of both husband and wife to the Human Sperm Bank for examination and retention of copies. All applications for self-sperm cryopreservation must fill out an application form and sign an informed consent form. Applicants for testicular tissue and sparse sperm cryopreservation must provide a diagnostic certificate from a hospital qualified in assisted reproductive technology, and also need to undergo laboratory tests for blood type, sexually transmitted diseases and other items. The main purpose of these examinations is to maintain your reproductive safety and avoid ethical disputes in the future. How to charge for testicular tissue and sparse sperm cryopreservation? Testicular tissue and sparse sperm cryopreservation belongs to the sperm bank’s self-sperm preservation business, and is charged according to the self-sperm preservation fee standard. In recent years, China’s human sperm bank semen cryopreservation technology has been developed very maturely, which largely reduces the damage of sperm in the ultra-low temperature environment. Especially for patients with oligozoospermia, weak spermatozoa and azoospermia, human sperm bank will do its best to retain the patient’s greatest hope for fertility. The Human Sperm Bank is motivated to contribute to the preservation of human fertility and to seek greater benefits for patients with self-sperm preservation.