The lesion is usually obtained by puncture biopsy, and the lesion is diagnosed as malignant according to the cellular morphology of the lesion under the microscope. The puncture biopsy is an invasive test used in the clinical diagnosis and treatment of diseases, and there may be certain risks such as bleeding, pain, infection, etc. 1. Bleeding: Since puncture biopsy is an invasive test, risks such as bleeding from the puncture site may occur. If local pressure is not performed according to medical advice, it may cause excessive bleeding or the formation of a hematoma, etc. Therefore, bed rest and pressure should be performed according to medical advice after puncture biopsy. If the local swelling or bright red bleeding visible to the naked eye is accompanied by chills, dizziness and difficulty in breathing, the doctor should be called immediately for disposal. 2. However, due to different tolerance ability and puncture site, the reaction to postoperative pain is different. Generally, the pain of superficial tumor puncture biopsy can be relieved in 6-8 hours after surgery. If the location is deeper or requires multiple biopsies, oral painkillers can be taken under the guidance of doctors to relieve the pain; 3. 4.Other: There may be related organ and tissue damage, cancer cell dissemination, anesthesia risk, and pneumothorax during lung biopsy, etc. Mild pneumothorax can be treated with proper rest, but severe pneumothorax needs closed chest drainage as soon as possible to relieve symptoms. However, in general, there are no adverse effects, and the clinical diagnosis and treatment value is high, and the need for examination exists. Its diagnostic value far outweighs the potential risk of harm and can be ignored to a certain extent. If puncture biopsy is required, patients should actively cooperate with medical workers to prevent unnecessary risks due to misuse caused by patients’ lack of cooperation.