IVF refers to in vitro fertilization, non-invasive DNA refers to non-invasive DNA prenatal testing, and there is no such thing as using non-invasive DNA prenatal testing with caution for IVF, it is recommended to use non-invasive DNA prenatal testing with caution for IVF populations such as multiple pregnancies and severe obesity. Non-invasive DNA prenatal testing is mainly used to sequence the fetal free DNA contained in the peripheral blood of pregnant women by extracting their venous blood and utilizing DNA sequencing technology. This test screens the fetus for chromosomal disorders such as Edward’s syndrome, Down’s syndrome, and Patau syndrome. Although IVF and natural pregnancies differ in terms of how the babies are conceived, the maternity tests are the same. However, it is recommended that pregnant women undergoing IVF who have multiple pregnancies, are severely obese, have chromosomal abnormalities, are over 35 years of age, or are at high risk during early or mid-term pregnancy testing should use non-invasive DNA prenatal testing with caution. Non-invasive DNA testing is available for most IVF, but not for pregnant women who are less than 12 weeks pregnant or have a combination of malignant tumors, transplants within 1 year, or allogeneic blood transfusions.