Most of the patients visit the clinic on an empty stomach, do not eat or drink in the morning, think that this facilitates the doctor to take clinical specimens, so that the urine bacterial culture may also need morning urine. However, the actual urine culture is not rigidly required to be morning urine, because each person’s pathology is different, some patients are a complex urinary tract infection, which will require him to send a specimen several times during the day, rather than simply morning urine. Some patients are seen in the morning and have not urinated or defecated in advance and have not eaten, so there is no problem in taking morning urine for bacterial culture at this time. So the requirements for retaining a urine specimen are to try not to use antibiotics and try not to drink a lot of water before sending it in for testing.