The percentage of gram-positive bacteria in the stool is usually <30%, positive bacteria include both cocci and bacilli, gram-positive bacilli percentage of 15% is usually normal. If the proportion of gram-positive bacilli is greater than 30%, it suggests that there may be intestinal flora dysbiosis. It is related to age, environment, diet, medication, infection, etc. Patients may have diarrhea, abdominal pain, abdominal distension, loss of appetite, etc. It is mostly seen in acute enteritis, after intestinal surgery, chronic colitis, intestinal mass, diabetes and other conditions. The treatment for intestinal dysbiosis is mainly exogenous supplementation of probiotics, such as bifidobacteria, lactobacilli, bacillus subtilis and so on. In addition to probiotic supplementation, allopathic and symptomatic treatment should be given, such as diarrhea patients can be combined with antidiarrheal agents such as montelukast, difenocoumarol, etc.; antibiotics such as norfloxacin, levofloxacin, etc. can be given in case of bacterial infections. For Gram-positive bacilli in 15% of cases, it is recommended to be clarified by the doctor in conjunction with clinical symptoms and other auxiliary examinations during the consultation.