Sperm DNA fragmentation test is an indicator used to evaluate sperm quality and predict male fertility. Sperm DNA fragmentation refers to the damage to sperm DNA caused by harmful factors (such as smoking, drinking, radiation, high temperature, staying up late, drugs, etc.) that may have affected the sperm during the formation process. As routine semen examination often cannot directly reflect the fertilization ability of sperm and its impact on embryo development, it is necessary for families with fertility disorders to go for sperm DNA fragmentation test. Sperm DNA Fragmentation Test requires checking the percentage of sperm with large halo, percentage of sperm with medium halo, percentage of sperm with small halo, percentage of sperm without halo, as well as sperm DNA fragmentation rate and sperm DNA integrity rate. These tests are used to evaluate the causes of male infertility and provide targeted treatment to solve the patient’s problems and improve the quality of fertility. Sperm DNA fragmentation test is mainly used for some infertility patients, men whose spouses have a history of habitual miscarriage, men who have poor lifestyle habits in general, and patients who plan to undergo IVF. It is necessary to listen to the doctor’s advice before the test, go to a regular hospital and have it performed by a professional doctor.