The purpose of a puncture biopsy is to clarify the diagnosis and guide the next step of treatment. Judging the benign and malignant nature of the disease based only on the imaging performance of the film is often prone to misdiagnosis. Even for patients with highly suspected tumors, pathological tests after puncture biopsy are needed to clarify the specific cytological type of the tumor, the degree of differentiation, the sensitivity of targeted drug therapy, and other relevant information to decide the treatment plan. A thin needle is usually punctured into the lesion with the help of fluoroscopy, CT, or ultrasound to obtain a tissue specimen, which is then observed under a microscope to clarify the diagnosis. The vast majority of tumor patients need to have puncture biopsy before receiving radiotherapy or chemotherapy treatment. Many patients and friends worry that the puncture will stimulate the tumor tissue and cause the tumor cells to grow like crazy, and some worry that it will make the tumor metastasize along the puncture tract, but according to our more than 10 years treatment experience, these situations have never occurred, so there is no need to worry.