Creatine kinase isoenzyme (CK) belongs to the cardiac enzyme spectrum, this enzyme exists in cardiac muscle or skeletal muscle, but the type CK-MB exists mainly in cardiac muscle cells, so clinically creatine kinase isoenzyme as long as it is higher than the normal reference value (CK-MB<0.05) basically represents myocardial injury. However, whether there is myocardial infarction, there are other markers to be tested such as troponin, if troponin is also significantly elevated, it may be myocardial infarction, if not, it may not necessarily be myocardial injury, it may be other muscle injury. The analysis should also be combined with clinical manifestations, electrocardiogram, echocardiogram and other subjective and objective evidence. If it is accompanied by chest pain and tightness during activity, middle-aged or elderly, or male, it should be considered as coronary heart disease or other heart disease, and if necessary, bed rest and hospitalization are needed to treat the cause. In conclusion, once elevated creatine kinase isoenzyme is found, the first thing to think about is to rule out heart disease. Of course, if the creatine kinase isoenzyme is elevated without any clinical symptoms or other elevated myocardial markers, heart disease or other causes can be ruled out and no special treatment is usually needed.