The five hepatitis B tests, are the more commonly used clinical tests. The actual early monitoring, mostly some qualitative monitoring, is useful to screen for hepatitis B virus infection, that is, positive surface antigen, or negative. In addition, it can also be used as a monitoring indicator for antibodies after hepatitis B vaccination. The five hepatitis B tests, mainly include surface antigen, surface antibody, E antigen, E antibody, and core antibody. The early ones were mainly a qualitative monitoring, that is, they could be designated as negative or positive. As the accuracy of reagents has improved, it has now progressed to a quantitative assay. Quantitative measurement has its own advantages, for example, surface antibody positivity can be divided into weak and strong positivity. After the hepatitis B vaccination, the surface antibody turns positive, which facilitates further management. If it is weakly positive, another booster may be needed. If it is strongly positive, no special treatment is needed, as the body has strong enough resistance, especially nowadays, during the anti-viral treatment of hepatitis B, the surface antigen can be gradually reduced due to the use of some interferon treatment programs. Therefore, the quantitative test among the five items of hepatitis B.