Sampling methods of lung nodules for pathologic examination mainly include bronchoscopy, lung puncture biopsy through the chest wall, thoracoscopy and so on. 1. Bronchoscopy: When doing the examination, the doctor needs to send the bronchoscope into the trachea from the patient’s mouth or nasal cavity, and then enter the lungs through the bronchial tubes, and take a few pieces of tissues from the nodule for pathological examination, which helps to diagnose the nature of the lung nodule. 2. Trans-thoracic wall lung puncture biopsy: for nodules caused by peripheral lung cancer, the sensitivity and specificity are higher, and puncture biopsy can be performed under CT guidance or fluoroscopy. 3. Thoracoscopic technique: it is suitable for patients who are unable to perform bronchoscopy or fail to undergo lung puncture biopsy through the chest wall, especially for tiny lung nodules. It is recommended that patients with lung nodules go to regular hospitals in time and listen to the advice of professional doctors.