Identifying the presence of liver fluke can be judged from the patient’s clinical manifestations, routine blood tests, liver function tests, pathogenetic tests, immunological tests, imaging tests and other aspects. 1. Clinical manifestations: patients may manifest abdominal pain and distension, diarrhea, fever, malaise, etc., mostly after eating raw fish or shrimp, patients may also have a history of living in infected areas or travel history. 2. Blood tests: patients may show a significant increase in the number of eosinophils, the total number of white blood cells can also be reduced, hemoglobin can also have a mild decline. 3. Liver function test: after infected with liver fluke, it may cause certain damage to the liver cells of the patients, and the levels of transaminase, γ-glutamyl transferase and alkaline phosphatase will be elevated. 4. Pathogenetic examination: If eggs are found in the patient’s feces or duodenal drainage fluid, it is the most direct way to confirm the diagnosis of liver fluke infection. 5. Immunological examination: If the patient is found to be positive for antibodies against Fasciola hepatica, it can also indicate the infection of Fasciola hepatica, but there is a high level of false positives, and it is usually used for epidemiological investigations and auxiliary clinical diagnosis. 6. Imaging examination: ultrasound, CT, magnetic resonance and other examinations can detect abnormalities of the liver or bile ducts, such as pericholangitis, hepatic abscess, bile duct dilatation and so on. If the patient suspects the existence of liver fluke infection, it is recommended to consult a doctor in time and improve the relevant examination under the guidance of the doctor to avoid delaying the condition.