Going for a methotrexate test after a late night is usually not elevated. Fetoprotein belongs to blood tumor markers in clinic, and its elevated value has a certain indication of primary liver cancer, but also can be seen in benign liver disease, gastrointestinal tract, reproductive tract, lung malignant tumors and so on. Simply staying up all night will not lead to the elevation of alpha-fetoprotein, but if you have the above mentioned tumors or liver lesions, the test may show elevation of alpha-fetoprotein regardless of whether you stay up all night. It is recommended that those who have abnormalities in this test should consult a doctor in time. Staying up late for a long period of time has a greater impact on health, which may lead to low immunity, aggravation of existing basic diseases, and easy occurrence of various cardiovascular and cerebrovascular diseases, etc. Those who have the habit of staying up late need to adjust their lifestyle in time.