With the development of technology, medical technology has also made great progress. At this stage, most diseases can be diagnosed and effectively treated in a timely manner. However, there are sometimes difficult cases encountered in clinical practice, where the diagnosis cannot be confirmed despite the use of advanced laboratory and imaging techniques. For tumor cases, sometimes the general direction of the tumor can be determined, but determining the specific type of tumor is decisive in choosing further treatment options, and then biopsy of the lesion can be chosen to help determine the diagnosis. Biopsies for urologic diseases are broadly divided into two main categories. One category, such as kidney, adrenal, prostate and retroperitoneal tumors, can be biopsied by puncture, which requires image-assisted targeting techniques, often with the help of ultrasound real-time targeting to help puncture biopsies, and more precise biopsies can be performed with the help of targeting techniques such as CT. Another category is diseases in the urinary tract, such as renal pelvis tumors, ureteral tumors, bladder tumors and urethral tumors, which can be detected and biopsied with the help of urethrocystoscopy, ureteroscopy and percutaneous nephroscopy techniques.