The preliminary results of this study showed that CXCR4 expression was not related to the age, gender, tumor size and growth mode of gastric cancer patients (p>0.05), but to the degree of tumor differentiation, depth of infiltration, lymph node metastasis and clinical stage (p<0.01), suggesting that CXCR4 expression is involved in the invasive metastasis of gastric cancer, and the detection of gastric cancer peritoneal metastatic tissues and metastatic lymphatic tissues The staining intensity expression of CXCR4 in gastric cancer tissues with local lymph node metastasis was significantly higher than that in gastric cancer tissues without local lymph node metastasis, and the difference was very significant (p<0.01), and it was hypothesized that CXCR4 combined with its ligand could inhibit the differentiation of gastric cancer cells and promote the growth and invasive metastasis of gastric cancer, associated with the specific high expression of SDF-l in lymph nodes [13], thus providing further support for the above view. This is also consistent with the expression of the chemokine receptor CXCR4 in other tumor tissues [14,15]. It has been reported that inhibition of chemokine receptor binding to its ligand through chemokine receptor antagonists can inhibit tumor growth and invasive metastasis [16]. Therefore, we suggest that antagonists of CXCR4 have the potential to be a new target for the treatment of gastric cancer by inhibiting the growth and invasive metastasis of gastric cancer. In this study, immunohistochemical results showed that as the lesions progressed: early gastric cancer → progressive gastric cancer → perigastric lymph nodes → peritoneal metastasis, the positive rate of CXCR4 expression showed a gradual upregulation, suggesting that gastric cancer cells expressing CXCR4 were more aggressive and metastatic. the expression of CXCR4 in primary tissues correlated with gastric cancer stage, tumor infiltration depth and lymph node metastasis, and CXCR4 is likely to It is likely that CXCR4 is an important molecule in the progression of gastric cancer, lymph node metastasis of gastric cancer, especially peritoneal invasion and metastasis [12]. The present study showed higher expression of CXCR4 in gastric cancer cells that developed metastasis, which further supports the idea that homing mechanism influences target organ selection. One study found [15] that approximately 4/5 of colon cancer tissues also expressed CXCR4 and that CXCR4 expression was closely associated with lymph node metastasis in colon cancer. The movement of CXCR4-positive gastric cancer cells to lymph nodes may have some similarities with the migration of lymphocytes, and therefore the detection of chemokine receptor CXCR4 expression in gastric cancer specimens can help predict the local lymph node metastasis of gastric cancer and determine the surgical extent of lymph node dissection. However, the role of upregulated CXCR4 expression in peritoneal invasive metastasis of gastric cancer and its related molecular mechanisms deserve further in-depth study.