Intermittent chills are one of the symptoms of malaria, an infectious disease caused by Plasmodium, characterized by intermittent chills, high fever, sweating, and splenomegaly and anemia. Cutting off the transmission route is mainly to eliminate Anopheles mosquitoes and prevent them from biting. Removal of Anopheles larvae breeding sites and use of insecticidal drugs. Personal protection can apply repellents or mosquito nets to avoid being bitten by mosquitoes. Control the source of infection, sound outbreak reporting, eradication of malaria patients with current symptoms and those with malaria parasites. Intermittent chills examination: 1, blood pathogen examination of the human four kinds of malaria parasite only falciparum malaria one in the peripheral blood only see ring body and gametocyte, and in the seizure period to detect the opportunity to be more, most of the protozoa into the visceral capillaries in the intermittent period of the seizure, such as at that time the gametocyte has not yet appeared, then the blood test may be temporarily negative, therefore, falciparum malaria in the seizure of the blood test during the most appropriate; the rest of the three kinds of malaria blood test is not limited by time The other three types of malaria are not limited by time, and the protozoa can be seen in both the attack and intermittent periods. Clinically malaria-like, blood test negative for protozoa, should adhere to the blood examination twice a day for a few days. Carefully check the thick blood film as prescribed, its power is many times higher than that of the thin blood film, and in all cases of malaria, the malarial parasite will eventually be detected in the peripheral blood. Smearing, staining, and microscopic examination of blood pricked from the patient’s earlobe or fingertip is still the most reliable method of confirming the diagnosis of malaria, and the diagnosis can be confirmed if Plasmodium intracellularis is found. In view of the fact that the accuracy of microscopic examination is affected by the density of protozoa in the blood, preparation and staining techniques, deformation of protozoa or decrease in density after drug administration, and microscopic examination experience, some improvements have been made in recent years to the traditional blood test method. One of them is Becton Dickinson’s QBC method (quantitative buffy coat). Using a capillary tube containing anticoagulant and acridine orange, 60μl of blood is taken from the patient, and a floater is added. After centrifugation, the plasmodium parasites are concentrated in the upper layer of red blood cells and the lower layer of white blood cells, and due to the presence of the floater in the center of the tube, the two layers of cells and the plasmodium parasites are pushed towards the wall of the tube, so that the plasmodium parasites which fluoresce can be examined under the fluorescence microscope directly. This method has a concentration effect, which improves the sensitivity and saves time by not requiring staining. The second is 0,5%~1,0% saponin solution instead of ordinary water hemolysis, and then stained with Giehl’s solution after microscopic examination. Advantage is that the thick blood film treated with saponin is clear, no red blood cell debris and platelet interference, which helps to detect Plasmodium. 2, immunological detection ① detection of malaria parasite antigen can be found out protozoaemia, so the clinical diagnosis of patients with current symptoms and from the population to check the source of infection, assessment of the efficacy of treatment can be used. The main methods include agarose diffusion test, convection immunoelectrophoresis, enzyme-linked immunosorbent assay, direct fluorescence or enzyme immunostaining. ② detection of Plasmodium antibodies can be used for epidemiological investigations, tracing the source of infection; with the help of determining the level of antibody levels of the population in endemic areas to infer the trend of malaria epidemics; screening blood donors to prevent malaria transfusion infections, as well as assessing the effectiveness of anti-malaria measures, etc. In addition, for repeated episodes of unidentified causes, the main method is to prevent malaria. In addition, malaria antibody testing can help to diagnose multiple episodes of unexplained malaria. Antibody detection methods more commonly used are indirect fluorescent antibody test, indirect hemagglutination test, enzyme-linked immunosorbent assay. 3.Nucleic acid probe test At present, there are several different kinds of nucleic acid probes used for malaria parasite detection at home and abroad. Due to its unique high specificity, sensitivity can be higher than the microscopic examination, it is believed that nucleic acid probe technology is very promising to replace the conventional microscopic examination, and can be processed in batches of large numbers of samples in a short period of time, it has been considered to be able to quantify and estimate the level of Plasmodium blood, is a very potential diagnostic tool for malaria epidemiological investigations and evaluating the effect of anti-malaria measures. There are still some technical problems to be solved in the mass production of nucleic acid probes and their large-scale use in the field. It is recognized that PCR has the highest sensitivity and specificity among the various malaria detection methods. In order to further improve the sensitivity and specificity of PCR technology, as well as to facilitate the promotion of practical work, on this basis, and nested PCR (nested PCR), PCR-ELISA and other methods of research. In addition to being able to directly detect Plasmodium in anticoagulated blood samples, the technique of PCR for detecting Plasmodium on dried blood droplets on filter paper has also matured, thus facilitating the use of PCR to monitor malaria in remote areas. Its application in the field is limited by the high demands on laboratory techniques and conditions. At present, in most malaria areas, after collecting blood on site, it is still necessary to return to the laboratory with better conditions for further analysis and processing. Currently, the World Health Organization recommends the application of Dipstick method, the principle of which is to use P. falciparum to synthesize and secrete a stable water-soluble antigen – histidine rich proteinII (HRPII), and then drop the monoclonal antibody prepared by P. falciparum on immunochromatographic strips, and then detect the histidine rich protein in blood after adsorption, washing and color development. After adsorption, washing and color development, the presence of histidine rich protein II in blood was detected. According to the reports of foreign countries comparing Dipstick and other methods, the sensitivity (84.2%~93.9%) and specificity (81.1%~99.5%) of Dipstick method for malaria diagnosis are higher; and it has the characteristics of simple operation, fast and stable, easy to learn, and it is suitable for the microscopic examination or the quality of the laboratory technology is difficult to ensure, and the malaria epidemiological range is to be determined, the malaria is in a low degree of transmission, and it is necessary to avoid drug abuse to detect the presence of rich histone II in the blood. It is suitable for areas where the epidemiological extent of malaria has to be determined, where malaria transmission is low, and where drug abuse has to be avoided to minimize the development of resistance. It is important to note that the Dipstick method has some limitations, as it is difficult to detect P. falciparum in the incubation period or when only mature gametocytes are present in the blood.