Having intercourse for 2 consecutive days can affect sperm quality. The sperm production cycle is about 76 days, and the seminal vesicle is the organ that stores sperm, which can be replenished quickly after the first ejaculation. It can be concluded that these are the same batch of sperm produced with the same quality. However, sperm quality has to be evaluated comprehensively and is not influenced by a single factor, which affects it, such as liquefaction time, total number of sperm, viability and sperm motility. The quantity of the second ejaculation will decrease compared to the first one, the total number of sperm will decrease, and the volume of semen will also decrease. In this case, the sperm quality will be affected, so in clinical practice, patients are required to abstain from sex for 3-5 days to ensure the authenticity and accuracy of the test.