The effect of tacrolimus in lowering urinary protein depends on the patient’s clinical symptoms and pathological manifestations, pathological manifestations of membranous nephropathy, microscopic lesions, etc., the use of tacrolimus, the effect of lowering urinary protein is still good; some patients applying tacrolimus in lowering urinary protein is not very good, and need to comply with the doctor’s instructions to replace it with hormone plus cyclophosphamide or rituximab and so on. Tacrolimus is mainly used in renal transplantation, anti-rejection reaction; renal disease is commonly used in membranous nephropathy, focal segmental sclerosing nephritis, microscopic lesion nephropathy and so on. In terms of lowering urinary protein, it is necessary to analyze according to the specific condition, for patients with larger amount of urinary protein and heavier condition, the effect is general, and generally clinically replaced with cyclophosphamide, rituximab, etc.; if the patient has less proteinuria and lighter type of pathology, tacrolimus is generally available as a treatment option, and the effect is still good. In addition, it is forbidden for those who are allergic to tacrolimus. Patients with kidney disease, even if the use of the drug, should be careful to use under the guidance of a doctor, and pay attention to monitoring renal function during the use of the drug. Tacrolimus also has adverse reactions in various systems, such as gastrointestinal reactions: diarrhea, nausea, vomiting, etc.; cardiovascular reactions: hypertension, blood clots, etc.; nervous system: tremor, headache, etc.. Patients who need to use tacrolimus need to go to a regular hospital, under the guidance of a physician to standardize the application.