Application: ①Diagnosis of Graves’ disease and identification of the cause of hyperthyroidism: the positive rate of TRAb in Graves’ disease is as high as 85.7%, while other causes of hyperthyroidism are generally negative. Therefore, TRAb can be used as a specific indicator of Graves’ disease. ②Judgment of efficacy and prognosis: TRAb is a reference indicator for judging efficacy and prognosis of immune remission in the course of antithyroid drug treatment. If TRAb is negative after treatment with antithyroid drugs, the drug should be stopped; TRAb is positive, indicating that the disease is prone to relapse after stopping the drug. ③Predicting neonatal hyperthyroidism: Since TRAb can be transported across the placenta, TRAb-positive pregnant women can cause neonatal hyperthyroidism. ④ Identify the cause of hypothyroidism: If a patient with hypothyroidism is accompanied by TRAb positivity, the hypothyroidism is caused by thyroid stimulating blocking antibodies.