We often joke always say idle “egg pain”, but what is specific egg pain, many people may have no concept. Mr. Lejia’s “egg story” is also a vivid lesson for us. In fact, in clinical practice, there is indeed a kind of surgery that makes us feel a bit “egg-ache” – testicular puncture. What is a testicular puncture? Testicular puncture is performed when a man does not see sperm on multiple semen tests (usually three), in order to clarify the status of sperm production in the testicles and help find the cause. Testicular aspiration is a type of testicular biopsy, mainly for patients with azoospermia. It is both a diagnostic and therapeutic clinical technique, which involves surgically removing a small piece of testicular biopsy to understand the spermatogenesis of the testes according to the testicular tissue structure and germ cells. Who needs a testicular puncture? At present, the examination methods to understand the spermatogenic function of the testis include semen examination, hormone examination, biochemical examination of seminal plasma and ultrasound examination, etc. However, if no spermatozoa are seen in the semen examination, none of these examination methods can directly reflect the spermatogenic function of the testis compared with testicular biopsy. This is because testicular biopsy is a direct examination of the varicocele of the testis, while endocrine and biochemical examinations are an indirect way to understand the spermatogenic function. Currently, testicular biopsy is the gold standard for diagnosing testicular spermatogenesis, so testicular puncture can be performed in patients with azoospermia if the cause is unknown and the testicular volume is still acceptable (usually at least greater than 6 ML). If the puncture has sperm, then one can try to have their own child using in vitro fertilization (ICSI). In some patients, even if obstructive azoospermia is highly suspected, such as a history of previous fertility and later azoospermia due to a history of epididymitis or vasectomy, an early puncture will be performed to understand testicular spermatogenesis before vas deferens-vas deferens or vas deferens-epidididymal microanastomosis. After all, there is no point in microanastomosis if there is a subsequent impairment of spermatogenesis in the testis, such as testicular damage, resulting in the testis not producing sperm anymore. Can testicular puncture damage the testicles? In addition to the fear of egg pain, many people are also concerned about whether testicular puncture is harmful. Can testicular puncture damage the testicles? Testicular puncture is the puncture of the testicles under local anesthesia with a medical puncture needle and syringe, performed in a sterile environment, and a small amount of testicular tissue is extracted, which is then placed under a microscope to observe the presence of sperm. If there are no sperm, pathological tests are usually also sent to further understand the tissue within the testicle. In fact, under normal circumstances, there are about 300 to 1,000 varicocele in a man’s testicles, and these varicocele are mainly connected to the testicular output tubules by dozens of small tubes. If only a small amount of testicular tissue is extracted, there is no impact on the structure of the testicular tubules and the output ducts. However, patients should remember that the balls are after all a fragile aristocracy, without muscles or bones, and prone to egg pain. Therefore, since the puncture procedure is blind, very few people may develop testicular hematoma due to touching the blood vessels in the testicle, so it is necessary to press the testicle on the punctured side for at least 30-40 minutes after the procedure. After surgery, the wound should be dry and treated with oral antibiotics. If a mild hematoma occurs, reduce activity and rest quietly, usually in about half a month, the hematoma will be gradually absorbed and the pain will be gradually relieved; however, if the hematoma gradually increases, it is recommended to seek emergency medical attention and if necessary, testicular incision is needed to stop the bleeding. Generally, as long as you pay attention to pressure rest after the operation and follow the doctor’s words, the probability of the above situation is small. What do I need to do before the puncture? Although the testicular puncture is not a major operation, it is an invasive operation, and the location is so critical for the majority of gay men, so some preparations should be made before the operation to reduce the risk of damage to the balls. First of all, some blood biochemical tests need to be completed to understand whether the patient has inflammation, abnormal blood clotting or infectious diseases to avoid bleeding from the balls. At the same time, it is generally necessary to prepare the skin before surgery, that is, to shave the pubic hair of the perineum, so as to facilitate the operation of the doctor on the one hand, and reduce the risk of wound infection on the other.