Urine protein two plus is very serious, it is recommended to actively treat, because there is no protein in normal human urine, only when there is kidney disease, it will lead to urine protein two plus. Because urine protein two plus without active treatment will lead to further aggravation of the disease, which can lead to renal insufficiency or even renal failure in severe cases, affecting the quality of survival and the survival time of patients. Causes of positive urine protein, such as primary kidney disease or secondary kidney disease, primary kidney disease including glomerulonephritis, nephrotic syndrome, etc., secondary kidney disease including diabetic nephropathy, hypertensive kidney damage, tumor-associated kidney disease, myelomeningocele kidney disease, etc. The amount of urine protein can be quantified by 24hUP, and renal puncture biopsy can be performed if necessary to clarify the type of kidney pathology, and secondary kidney disease requires active treatment of the primary disease.