Fecal transplantation is simply the extraction of functional flora from the feces of a healthy person and transplantation into the intestine of the patient for the purpose of rebuilding the patient’s new intestinal flora and thus achieving the treatment of internal and external intestinal diseases. In fact, the earliest record about fecal transplantation is in 300-400 AD, our ancestor Ge Hong recorded in a medical book during the Eastern Han Dynasty that he used human fecal supernatant to treat food poisoning and severe diarrhea, and achieved relatively good results. In 1958, a surgeon in the United States treated pseudomembranous enteritis with fecal transplantation and achieved good results in Western countries. With the development of the times, the methodology of fecal transplantation has also evolved, from the most primitive manual production method to the modern intelligent machine separation, which is now the standardized fecal transplantation. The so-called standardized fecal transplantation is a highly purified isolation of the flora from the feces of a healthy person with the help of modern laboratory instruments, which is then transplanted into the intestinal tract of the patient. Standardized fecal isolation requires high hardware and software requirements. First, there must be an independent fecal isolation laboratory with trained and experienced operating technicians, and second, there must be a relatively complete and rigorous donor screening system.